HORDEIN GENE DOSE EFFECTS IN TRIPLOID ENDOSPERM OF BARLEY (Hordeum vulgare L.)
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منابع مشابه
Hordein gene expression in a low protein barley cultivar.
The low protein barley (Hordeum vulgare L.) cultivar ;Karl' is deficient in hordeins, the major storage protein fraction, but has normal levels of other endosperm protein fractions. We compared hordein gene expression in Karl with that in ;Traill,' a related cultivar that contains normal amounts of hordein. In plants grown in controlled environmental conditions, hordein accumulation in Karl was...
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The B-hordeins are the major group of prolamin storage proteins in barley (Hordeum vulgare L.) and they are encoded by a small multigene family that is expressed specifically in the developing endosperm. We report the complete nucleotide sequence of a clone of one B-hordein gene (pBHR184). The cloned gene contains no introns and belongs to the B1 sub-family of B-hordein genes. Comparison of the...
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ADP-glucose pyrophosphorylase (AGPase), a two-gene-encoded enzyme, is the key component of starch synthesis in all plants. In the present study, we have used an E. coli expression system for the (over)production of proteins derived from both full length and specifically truncated cDNAs encoding small subunits of AGPase from seed endosperm (AGPase-B1) and leaves (AGPase-B2) of barley (Hordeum vu...
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Soluble products, released by the hydrolysis of hordeins into the media of barley (Hordeum vulgare cv. Perth) half-seeds were analyzed. Large polypeptide fragments (methanol-insoluble) were identified using the Western immunoblot technique with the antibodies prepared against B and C polypeptides of hordein. A number of hordein IgG-reacting bands were noted in the samples from dry kernels. In s...
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